hFGFR3-G380R
系統名
C57BL/6JSmo-Fgfr3tm1(hFGFR3-G380R)Smoc
SMOC番号
NM-HU-232149
維持形態
Sperm cryopreservation
遺伝子の概要
Gene Symbol
Fgfr3
説明
表現型データ
Fig.1 Detection of FGFR3 expression in knee joint and brain in 2.4-week-old male and female hFGFR3-G380R knockin mice by RT-PCR.
HepG2 cells were used as the WT control of human FGFR3 RNA.
Wild type: one band at 217 bp with primers F1/R1(mFgfr3) and one band at 123 bp with primers F/R(mGapdh);
Heterozygous: one band at 217 bp with primers F1/R1(mFgfr3), one band at 375 bp with primers F2/R2(hFGFR3) and one band at 123 bp with primers F/R(mGapdh).
Abbr. M, DNA marker; HE, heterozygous; WT, wild type.
Fig.2 Sequencing analysis of human FGFR3 mRNA in HepG2 cells and hFGFR3-G380R knockin mice.
Sequencing data of RT-PCR products confirmed the presence of a G to A mutation in human FGFR3 mRNA expressed in hFGFR3-G380R knockin mice.
Abbr. HE, heterozygous.
Fig.3 Detection of human FGFR3 expression in 2.4-week-old male and female hFGFR3-G380R knockin mice by WB.
Abbr. M, marker; WT, wild type; HE, heterozygous; PC, positive control, HepG2 cells.
Note. The anti-human FGFR3 antibody cross-reacted with mouse FGFR3 and humanized FGFR3.
Fig.4 Body weight of WT C57BL/6 and heterozygous hFGFR3-G380R knockin mice. (n=1)
Fig.5 Micro-CT analysis of skeleton in 35-day-old WT C57BL/6 and heterozygous hFGFR3-G380R mice.
Fig.6 Micro-CT analysis of skeleton in 49-day-old WT C57BL/6 and heterozygous hFGFR3-G380R mice.
Fig.7 Micro-CT analysis of skeleton in 63-day-old WT C57BL/6 and heterozygous hFGFR3-G380R mice.
Fig.8 Micro-CT analysis of skeleton in WT C57BL/6 and heterozygous hFGFR3-G380R mice (n=1).
Fig.9 Micro-CT analysis of femur in 35-day-old WT C57BL/6 and heterozygous hFGFR3-G380R mice.
Fig.10 Micro-CT analysis of femur in 49-day-old WT C57BL/6 and heterozygous hFGFR3-G380R mice.
Fig.11 Micro-CT analysis of femur in 63-day-old WT C57BL/6 and heterozygous hFGFR3-G380R mice.
Fig.12 Micro-CT analysis of femur in WT C57BL/6 and heterozygous hFGFR3-G380R mice (n=1).
Fig.13 Micro-CT analysis of skull in 35-day-old WT C57BL/6 and heterozygous hFGFR3-G380R mice.
Fig.14 Micro-CT analysis of skull in 49-day-old WT C57BL/6 and heterozygous hFGFR3-G380R knockin mice.
Fig.15 Micro-CT analysis of skull in 63-day-old WT C57BL/6 and heterozygous hFGFR3-G380R knockin mice.
Fig.16 Micro-CT analysis of skull in WT C57BL/6 and heterozygous hFGFR3-G380R mice (n=1).
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