Nkx2-5-IRES-Cre

系統名

C57BL/6Smoc-Nkx2-5em1(IRES-Cre-WPRE-polyA)Smoc

SMOC番号

NM-KI-200152

維持形態

Sperm cryopreservation

PDFを取得

遺伝子の概要

Gene Symbol
Nkx2-5

Synonyms

Csx; Nkx2.5; tinman; Nkx-2.5

NCBI ID

18091

MGI ID

97350

Ensembl ID

ENSMUSG00000015579

Pubmed

Nkx2-5

Human Ortholog

NKX2-5

説明

A IRES-Cre-WPRE-polyA expression cassette was knocked into the Nkx2-5 gene stop codon site.
応用分野：Cre recombinase tool; Nkx2.5 acts as a key transcription factor and plays essential roles for heart formation. By mating the reporter mice with Cre-expressing mice, Nkx2-5 positive cells derived from double-positive mice were permanently labeled by fluorescent protein. When crossed with mice carrying a targeted floxed alleles, this strain is useful in eliminating tissue-specific conditional expression of the gene.

表現型データ

image.png

Fig. 1 Cre-mediated recombination in the heart of Nkx2-5Cre/+; Rosa26tdTomato/+ mouse. TdTomato(red) expression can be detected in the myocardial cells and vascular cells of Nkx2-5Cre/+; Rosa26tdTomato/+ mouse.

image.png

Fig. 2 Detection of tdTomato(red) in various tissues of Nkx2-5Cre/+; Rosa26tdTomato/+ mice. Cre mediated recombination can be detected in the heart, bronchus, pulmonary alveoli and submandibular gland. Tdtomato expression can be also detected in individual cells derived from the brain, retina, skin, kidney, pancreas, intestine, colon, spleen and thymus, except for pituitary and muscle. (For more detailed information please contact our technical advisor.)


あなたも好きかも

Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute

On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.

詳細
Milestones

Our vision is to provide researchers all over the world with comprehensive, convenient and professional animal model services to facilitate a simplified and highly-efficient approach towards uncovering the mysteries of life.

詳細