hC5

系統名

C57BL/6Smoc-Hcem1(hC5)Smoc

SMOC番号

NM-HU-2000013

維持形態

Repository Live

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遺伝子の概要

Gene Symbol

説明

The endogenous mouse C5a gene was replaced by human C5 gene.
応用分野:Immunotherapy,cancer research,drug screening

表現型データ

image.png

Fig 1. Detection of C5 expression by RT-PCR. 

Wild type: only one band at 167 bp with primers F2/R2(mC5);

Homozygous: only one band at 337 bp with primers F1/R1(hC5).

Abbr. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

image.png

Fig 2. Analysis of humanized C5 and humanized C5a expression in serum in the humanized C5 mouse by ELISA. 

The homozygous hC5 KI mice were treated with LPS or NS (3mg/kg, i.p.) for 6 hours.

image.png

Fig 3. Detection of hC5 expression in eyes in hC5 KI mice by IF (n=3/group). 


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