Il2-IRES-Venus-Luc

系統名

C57BL/6Smoc-Il2em1(Venus-Luci)Smoc

SMOC番号

NM-KI-18057

維持形態

Embryo cryopreservation

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遺伝子の概要

Gene Symbol
Il2

説明

an IRES-Venus Luci cassette was inserted into the IL2 gene stop codon site.
応用分野:Gene tracing

表現型データ

il2-luci-1.png

Fig1.Hepa1-6 tumor volumes in Il2-Venus-Luci reporter mice treated or not with anti-PD-1 mAb. Arrows indicate treatment days.

il2-luci-2.png

Fig2. In vivo imaging validation of  Hepa1-6  tumors in Il2-Venus-Luci reporter mice treated or not with anti-PD-1 mAb.

In PBS treated group, the IL2 signal increased as the tumor development, which indicated an inflamed immune environment. In the PD1 mAb treated group, the IL2 signal is enhanced, especially at day 21.

il2-luci-3.png

Fig3. IL2 signal in ventral position of Il2-Venus-Luci reporter mice.

IL2 signal from abdomen is higher in PD1 treated mice than control mice on day 21. The signal should be produced by immune cells in mesenteric lymph nodes.

il2-luci-4.png

Fig4. Tissue IL2 signal in the Il2-Venus-Luci reporter mice at termination.

Tumor draining lymph node(TDLN) from PD1 treated mice have significantly higher IL2 signal than control mice. While tumor and spleen showed lowered or comparable IL2 signal compared to control mice.

The data is provided by Crownbio.


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