hPSGL-1(2)

系統名

C57BL/6Smoc-Selplgem2(hSELPLG)Smoc

SMOC番号

NM-HU-210026

維持形態

Embryo cryopreservation

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遺伝子の概要

Gene Symbol
Selplg

説明

The endogenous mice Selplg gene was completely or partially replaced by human SELPLG gene via CRISPR/Cas9 mediated recombination.
応用分野:inmmue therapy;drug screening

表現型データ

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Fig1. Detection of PSGL-1 expression in thymus and spleen by RT-PCR. Wild type: only one band at 248 bp with primers F1/R1(mPsgl-1);Homozygous: only one band at 237 bp with primers F2/R2(hPSGL-1); Abbr.. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

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Fig2. Detection of hPSGL-1 expression on B cells in blood in hPSGL-1 knockin mice.

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Fig3. Detection of hPSGL-1 expression on Monocytes and Granulocytes in blood in hPSGL-1 knockin mice.

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Fig4. Detection of hPSGL-1 expression on B cells in bone morrow in hPSGL-1 knockin mice.

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Fig5. Detection of hPSGL-1 expression on Monocytes and Granulocytes in bone morrow in hPSGL-1 knockin mice.

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Fig6. Detection of hPSGL-1 expression on T cells in bone morrow in hPSGL-1 knockin mice.

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Fig7. Detection of hPSGL-1 expression on NKs and DCs in bone morrow in hPSGL-1 KI mice.

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Fig8. Detection of hPSGL-1 expression on B cells in spleen in hPSGL-1 KI mice.

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Fig9. Detection of hPSGL-1 expression on Monocytes and Granulocytes in spleen in hPSGL-1 knockin mice.

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Fig10. Detection of hPSGL-1 expression on T cells in Thymus in hPSGL-1 knockin mice.


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Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute

On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.

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Milestones

Our vision is to provide researchers all over the world with comprehensive, convenient and professional animal model services to facilitate a simplified and highly-efficient approach towards uncovering the mysteries of life.

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