hPD-L2

系統名

C57BL/6Smoc-Pdcd1lg2em1(hPDCD1LG2)Smoc

SMOC番号

NM-HU-190050

維持形態

Embryo cryopreservation

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遺伝子の概要

Gene Symbol
Pdcd1lg2

Synonyms

Btdc; B7-DC; PD-L2; F730015O22Rik

NCBI ID

58205

MGI ID

1930125

Ensembl ID

ENSMUSG00000016498

Pubmed

Pdcd1lg2

Human Ortholog

PDCD1LG2

説明

将小鼠Pdcd1lg2基因全部或者部分替换为人源PDCD1LG2，从而表达人或人鼠嵌合PDCD1LG2蛋白，取代小鼠内源Pdcd1lg2蛋白的表达 hPD-L2.
応用分野：Immunotherapy,cancer research,drug screening

表現型データ

PD-L2-1.png

Fig1.Schematic representation of gene targeting at the Pd-l2 locus.

PD-L2-2.png

Fig2. Genotyping of humanized PD-L2 mice by RT-PCR.

Primer P1/2

Wild type = 404 bp

Primer P3/4

Mutant type = 405 bp

M:100bp Plus DNA ladder from Transgen (BM311)

PD-L2-3.png

Fig 3. Splenocytes from humanized PD-L2 mice were analyzed by flow cytometry after activation. 

hPD-L2+ (but not mPD-L2+) cells were detectable in the homozygous hPD-L2 mice, while hPD-L2+ and mPD-L2+ cells were both detectable in heterozygous humanized PD-L2 mice. 

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