hIL21/hIL21R

系統名

C57BL/6Smoc-Il21tm1(hIL21)Il21rtm1(hIL21R)Smoc

SMOC番号

NM-HU-220038

維持形態

Embryo cryopreservation

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遺伝子の概要

Gene Symbol
IL21

Synonyms

IL-21

NCBI ID

60505

MGI ID

1890474

Ensembl ID

ENSMUSG00000027718

Pubmed

IL21

Human Ortholog

IL21

Gene Symbol
IL21R

Synonyms

NILR

NCBI ID

60504

MGI ID

1890475

Ensembl ID

ENSMUSG00000030745

Pubmed

IL21R

Human Ortholog

IL21R

説明

hIL21(NM-HU-200255) were crossed with hIL21R(NM-HU-210031) to generate hIL21/hIL21R mice.

表現型データ

image.png

Fig 1. Detection of IL21 expression in splenocyte by RT-PCR. 

Wild type: only one band at 272 bp with primers F1/R1 (mIl21);

Homozygous: only one band at 304 bp with primers F2/R2 (hIL21); 

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

image.png

Fig 2. Detection of hIL21 expression in culture supernatants of splenocyte by ELISA (n=3). 

Abbr. HO, homozygous; WT, wild type.

Note. Splenocytes from C57BL/6 and homozygous hIL21/hIL21R dKI mice were stimulated with anti-mCD3 antibody in vitro.

image.png

Fig 3. Detection of IL21R expression on spleen-derived activated T cells and B cells in hIL21/hIL21R dKI mice.

Note. Splenocytes from C57BL/6 and homozygous hIL21/hIL21R dKI mice were stimulated with anti-mCD3 antibody in vitro.

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