hGLP1R

系統名

C57BL/6Smoc-Glp1rem2(hGLP1R)Smoc

SMOC番号

NM-HU-200220

維持形態

Repository Live

PDFを取得

遺伝子の概要

Gene Symbol
Glp1r

Synonyms

GLP-1R; GLP1Rc

NCBI ID

14652

MGI ID

99571

Ensembl ID

ENSMUSG00000024027

Pubmed

Glp1r

Human Ortholog

GLP1R

説明

The endogenous mouse Glp1r gene was completely or partially replaced by human hGLP1R gene via CRISPR/Cas9 mediated recombination.
応用分野：Diabetes research; drug screening

表現型データ

1.png

Fig1. Analysis of hGLP1R expression by IHC. Pancreata from wild-type and hGLP1R mice (HO) were stained using an antibody specific for human GLP1R, and only the pancreas from hGLP1R mice (HO) showed hGLP1R positive signal in pancreatic islet.

image.png

Fig2. Validation of the hypoglycemic efficacy of the test compound in humanized GLP-1R mice. The oral glucose tolerance test (OGTT) was performed (n=5), and the test compound showed a better hypoglycemic efficacy (p<0.001). The above data indicated that the GLP-1R humanized mouse is an effective model for diabetes drug screening.

image.png

Fig 3. Efficacy study of PF06882961 in hGLP1R mice. PF06882961 has shown significant effect in hGLP1R mice in OGTT study, but no effect in C57BL/6 mice.

image.png

Fig 4. Efficacy study of PF06882961 in HFD/STZ induced type II diabetes model in hGLPIR mice. PF06882961 could significant decreases the body weight(A); PF06882961 could significantly decrease fast blood glucose level and AUC in OGTT study (B).

image.png

Fig5. Detection of human GLP-1R expression in HO hGLP-1R knockin mice by IHC. Pancreas, salivary gland, colon, heart, lung, brian, stomach and duodenum were collected from homozygous hGLP-1R mice (HO/HO), and analyzed by IHC with anti-human GLP-1R antibody. Human GLP-1R is specifically expressed in islets of the pancreas. Abbr. HO/HO, homozygous.

Table1. Blood routine test results of Hom hGLP-1R mice (Data are presented as mean and ± SEM).

image.png

Table2. Biochemistry examinations results of Hom hGLP-1R mice (Data are presented as mean and ± SEM).image.png

あなたも好きかも

Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute

On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.

詳細
Customizing Mouse Models: Delivering in 100 Days to Speed Up Your Research!

At GenoBioTX, we understand that the lengthy wait times for gene-modified mouse models can hinder your research progress. Traditional methods often require 6-9 months, leading to delays and increased costs. That’s why we’re thrilled to introduce our innovative service designed to streamline this process and deliver results faster.

詳細