hPSMA
系統名
C57BL/6Smoc-Folh1em1(hPSMA-Wpre-PA)Smoc
SMOC番号
NM-HU-200240
維持形態
Embryo cryopreservation
遺伝子の概要
Gene Symbol
Folh1
説明
表現型データ
Fig1. Detection of PSMA expression in kidney by RT-PCR. Wild type: only one band at 294 bp with primers F1/R1(mPsma);Heterozygous: one band at 294 bp with primers F1/R1(mPsma) and one band at 169 bp with primers F2/R2(hPSMA); Abbr.. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.
Fig2. Detection of hPSMA expression in prostate in hPSMA KI mice.
Fig3. Analysis of human PSMA expression in the MC38-hPSMA cell line and the transplanted tumor of the PSMA humanized mice by FACS. Most of the MC38-hPSMA cells express human PSMA (A,B); the transplanted tumor formed by MC38-hPSMA cell line expresses human PSMA on the CD45- cells (C).(Completed in collaboration with CrownBio)
Fig4. Subcutaneous tumor formation experiment of the MC38-hPSMA cells in the PSMA humanized mice .The tumor volume was measured every 5 days after injection of MC38-hPSMA cells (n=10).The previous studies have shown that wild type mice would spontaneously reject the syngeneic tumors expressing an introduced human tumor antigen-hPSMA. While the MC38-hPSMA cells can form tumors subcutaneously in the humanized PSMA mice, indicating that the PSMA humanized mouse model can be used to transplant the MC38-hPSMA cell line.(Completed in collaboration with CrownBio)
Fig5. Detection of human PSMA expression in Prostate by IHC.
Note. The human PSMA antibody cross-reacted with mouse PSMA and human PSMA.
Abbr. HO, homozygous; WT, wild type.
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On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.
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