Uox-KO(2)

系統名

C57BL/6Smoc-Uoxem1Smoc

SMOC番号

NM-KO-191205

維持形態

Repository Live

PDFを取得

遺伝子の概要

Gene Symbol
Uox

Synonyms

AI663847

NCBI ID

22262

MGI ID

98907

Ensembl ID

ENSMUSG00000028186

Pubmed

Uox

Human Ortholog

UOX

説明

Uox-KO(2) mice(Stock No.NM-KO-191205）carry a knockout allele derived from the targeted deletion of exon 3-4.
応用分野：hyperuricemia research

病気の予測

nephrogenic diabetes insipidus

表現型

MGI:3687865

参考文献

Kelly SJ, Delnomdedieu M, Oliverio MI, Williams LD, Saifer MG, Sherman MR, Coffman TM, Johnson GA, Hershfield MS, Diabetes insipidus in uricase-deficient mice: a model for evaluating therapy with poly(ethylene glycol)-modified uricase. J Am Soc Nephrol. 2001 May;12(5):1001-9

表現型データ

image.png

Fig1. Construction of hyperuricemic model in Uox-ko mice. 

Uox-KO female mice were fed a synthetic diet containing allopurinol during pregnancy (Group 3), and the male offspring continued to be fed for 10 weeks after birth, with the levels of UA (uric acid), BUN (blood urea nitrogen), and body weight of the mice being measured.

image.png

image.png

Fig 2. Histological observation of kidney tissue in Uox-KO mice (HE & Masson staining). 

The results showed that the kidneys of Uox-KO mice exhibited interstitial fibrosis, inflammatory cell infiltration; glomerular atrophy, mesangial tissue proliferation; thickening of the vascular walls; dilation of renal tubules forming cysts; and homogeneous red-stained material exudation within the lumen of the renal tubules. Allopurinol (Group 3) was unable to ameliorate the kidney lesions caused by Uox-KO in mice. Uox-KO mice exhibit elevated uric acid levels and kidney damage similar to clinical conditions, and hyperuricemia treatment drugs can demonstrate the efficacy of lowering blood uric acid levels in these mice. Therefore, these mice can serve as a suitable model for the study of hyperuricemia and the evaluation of related drug effects.

image.png

Fig 3. Body Weight of Uox-KO mice.

image.png

Fig 4. Uric acid (UA) levels of Uox-KO mice. 

The results showed that the UA levels in Uox KO mice were significantly higher compared to the wild-type control mice.

あなたも好きかも

Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute

On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.

詳細
Customizing Mouse Models: Delivering in 100 Days to Speed Up Your Research!

At GenoBioTX, we understand that the lengthy wait times for gene-modified mouse models can hinder your research progress. Traditional methods often require 6-9 months, leading to delays and increased costs. That’s why we’re thrilled to introduce our innovative service designed to streamline this process and deliver results faster.

詳細