hMASP2

系統名

C57BL/6Smoc-Masp2em1(hMASP2)/Smoc

SMOC番号

NM-HU-2000076

維持形態

Repository Live

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遺伝子の概要

Gene Symbol
Masp2

Synonyms

MAp19; MASP-2

NCBI ID

17175

MGI ID

1330832

Ensembl ID

ENSMUSG00000028979

Pubmed

Masp2

Human Ortholog

MASP2

説明

The endogenous mouse Masp2 gene was replaced by human MASP2 gene.
応用分野：Immunotherapy,drug screening

表現型データ

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Fig1. Detection of MASP2 expression in liver by RT-PCR. 

Wild type: only one band at 479 bp with primers F1/R1(mMasp2);

Homozygous: only one band at 338 bp with primers F2/R2(hMASP2); 

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

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Fig2. Detection of hMASP2 expression in liver in HO hMASP2 mice by western blot.

Abbr. HO, homozygous; WT, wild type.

Note: The anti-human MASP2 antibody cross-reacted with mouse MASP2 and humanized MASP2.

image.png

Fig3. Detection of hMASP2 expression in liver in HO hMASP2 mice by IHC.

Abbr. HO, homozygous; WT, wild type.

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Fig4. Detection of hMASP2 expression in kidney in HO hMASP2 mice by IHC.

Abbr. HO, homozygous; WT, wild type.

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Fig5. Detection of IgA expression in liver in HO hMASP2 mice by IHC.

Abbr. HO, homozygous; WT, wild type.

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Fig6. Detection of IgA expression in kidney in HO hMASP2 mice by IHC.

Abbr. HO, homozygous; WT, wild type.

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Fig7. No obvious pathological features were found in liver in HO hMASP2 mice.

Abbr. HO, homozygous; WT, wild type.

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Fig8. No obvious pathological features were found in kidney in HO hMASP2 mice.

Abbr. HO, homozygous; WT, wild type.

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Fig9. Detection of mC3 expression in serum by ELISA. 

Abbr. HO, homozygous; WT, wild type.

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Fig10. Lectin and alternative pathway activity of HO hMASP2 mice. 

LP activity in serum of age-matched female (A) and male (B) wild-type C57BL/6 and HO hMASP2 mice at different dilutions (n=3/group); (C) AP activity in serum of age-matched male wild-type C57BL/6 and HO hMASP2 mice at different dilutions (n=3/group).

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Fig11. LNP023 blocks alternative complement pathway activity in the serum of age-matched male wild-type C57BL/6 and HO hMASP2 mice (n=3/group).

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