hGLP1R

系統名

C57BL/6Smoc-Glp1rem2(hGLP1R)Smoc

SMOC番号

NM-HU-200220

維持形態

Repository Live

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遺伝子の概要

Gene Symbol
Glp1r

説明

The endogenous mouse Glp1r gene was completely or partially replaced by human hGLP1R gene via CRISPR/Cas9 mediated recombination.
応用分野:Diabetes research; drug screening

表現型データ

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Fig 1. Detection of GLP1R and Gapdh expression in lung, heart, brain, stomach, salivary gland, duodenum and samll intestine by RT-PCR.

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Fig 2. Analysis of hGLP1R expression by IHC. Pancreata from wild-type and hGLP1R mice (HO) were stained using an antibody specific for human GLP1R, and only the pancreas from hGLP1R mice (HO) showed hGLP1R positive signal in pancreatic islet.

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Fig 3. Validation of the hypoglycemic efficacy of the test compound in humanized GLP-1R mice. The oral glucose tolerance test (OGTT) was performed (n=5), and the test compound showed a better hypoglycemic efficacy (p<0.001). The above data indicated that the GLP-1R humanized mouse is an effective model for diabetes drug screening.

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Fig 4. Efficacy study of PF06882961 in hGLP1R mice. PF06882961 has shown significant effect in hGLP1R mice in OGTT study, but no effect in C57BL/6 mice.

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Fig 5. Efficacy study of PF06882961 in HFD/STZ induced type II diabetes model in hGLPIR mice. PF06882961 could significant decreases the body weight(A); PF06882961 could significantly decrease fast blood glucose level and AUC in OGTT study (B).

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Fig 6. Detection of human GLP-1R expression in HO hGLP-1R knockin mice by IHC. Pancreas, salivary gland, colon, heart, lung, brian, stomach and duodenum were collected from homozygous hGLP-1R mice (HO/HO), and analyzed by IHC with anti-human GLP-1R antibody. Human GLP-1R is specifically expressed in islets of the pancreas. Abbr. HO/HO, homozygous.

Table1. Blood routine test results of Hom hGLP-1R mice (Data are presented as mean and ± SEM).

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Table2. Biochemistry examinations results of Hom hGLP-1R mice (Data are presented as mean and ± SEM).

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