hMAPT

系統名

C57BL/6Smoc-Mapttm1(hMAPT)Smoc

SMOC番号

NM-HU-200116

維持形態

Repository Live

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遺伝子の概要

Gene Symbol
Mapt

Synonyms

Tau; Mtapt; AI413597; AW045860

NCBI ID

17762

MGI ID

97180

Ensembl ID

ENSMUSG00000018411

Pubmed

Mapt

Human Ortholog

MAPT

説明

The endogenous mouse Mapt gene was replaced by human MAPT gene. While hMAPT(2)(Stock No.NM-HU-200257) mice function similarly to hMAPT mice,for more detailed information please contact our technical advisor.

表現型データ

image.png

Fig.1 Detection of MAPT expression in cerebellum by RT-PCR. 

Wild type: only one band at 200 bp with primers F1/R1 (mMapt); 

Homozygous: only one band at 181 bp with primers F2/R2 (hMAPT); 

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

image.png

Fig 2. Detection of mouse MAPT expression in Brain in hMAPT knockin mice by IHC. 

Brains were collected from wild-type mice (+/+) and homozygous hMAPT mice (HO), and analyzed by IHC with anti-human MAPT antibody. 

Abbr. HO/HO, homozygous; +/+, wild type. 

Note. The anti-mouse MAPT Antibody cross-reacted with mouse MAPT and humanized MAPT.

image.png

Fig 3. Detection of human MAPT expression in Brain in hMAPT knockin mice by IHC. 

Brains were collected from wild-type mice (+/+) and homozygous hMAPT mice (HO), and analyzed by IHC with anti-human MAPT antibody. 

Abbr. HO/HO, homozygous; +/+, wild type. 

Note. The anti-human MAPT Antibody cross-reacted with mouse MAPT and humanized MAPT.

image.png

Fig 4. Detection of human MAPT expression in Brain in hMAPT knockin mice by IF. 

Brains were collected from wild-type mice (+/+) and homozygous hMAPT mice (HO), and analyzed by IF with anti-human MAPT antibody. 

Abbr. HO/HO, homozygous; +/+, wild type. 

Note. The anti-human MAPT Antibody cross-reacted with mouse MAPT and humanized MAPT.

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