R26-CAG-2xKRAB-dCas9-IRES-EGFP
系統名
C57BL/6Smoc-Gt(ROSA)26Sorem1(CAG-Loxp-2xKRAB-dCas9-IRES-EGFP)Smoc
SMOC番号
NM-KI-18007
維持形態
Sperm cryopreservation
遺伝子の概要
Gene Symbol
Gt(ROSA)26Sor
説明
These mice harbor a CAG-LSL-2xKRAB-dCas9-IRES-EGFP-WPRE-pA conditional expression cassette in mouse Rosa26 loci via homologous recombination. This strain can be used in CRISPR mediated target gene transcriptional inhibition (CRISPRi). The loxp-stop-loxp cassette was deleted.
応用分野：CRISPRi,gene regulation tool
*Literature published using this strain should indicate: R26-CAG-2xKRAB-dCas9-IRES-EGFP mice (Cat. NO. NM-KI-18007) were purchased from Shanghai Model Organisms Center, Inc..
表現型データ
Validation of 2xKRAB-dCas9 expression by Western blot
Rosa26- CAG-LSL-2xKRAB-dCas9 mice were crossed with Dppa3-Cre mice to delete STOP cassette.
Validation of EGFP expression by fluorescence assay
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Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute
On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.
詳細
Milestones
Our vision is to provide researchers all over the world with comprehensive, convenient and professional animal model services to facilitate a simplified and highly-efficient approach towards uncovering the mysteries of life.
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