hCSF1/hCSF1R(BALB/c)

系統名

BALB/cAnSmoc-Csf1tm2(CSF1)Csf1rem3(hCSF1R)/Smoc

SMOC番号

NM-HU-220016

維持形態

Repository Live

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遺伝子の概要

Gene Symbol
CSF1

Gene Symbol
CSF1R

説明

The endogenous mouse Csf1 and Csf1r genes was replaced respectively by human CSF1 and CSF1R genes.

表現型データ

image.png

Fig.1 Detection of CSF1 expression in spleen and lung by RT-PCR. 

Wild type: only one band at 299 bp with primers F1/R1(mCsf1);

Homozygous: only one band at 261 bp with primers F2/R2(hCSF1); 

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

image.png

Fig.2 Detection of CSF1R expression in spleen and lung by RT-PCR. 

Wild type: only one band at 233 bp with primers F1/R1(mCsf1r);

Homozygous: only one band at 345 bp with primers F2/R2(hCSF1R); 

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

image.png

Fig.3 Detection of hCSF1 expression in serum by ELISA (n=3). 

Abbr. HO, homozygous; WT, wild type.

Note. hCSF1/hCSF1R and BALB/c mice were i.p. injected with LPS for 6h.

image.png

Fig.4 Expression characterization of hCSF1R on Monocytes/ macrophages in Bone marrow in hCSF1/hCSF1R dKI mice.


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