Cd8a-2A-tdTomato-2A-Luc

系統名

C57BL/6Smoc-Cd8aem(T2A-tdTomato-P2A-Luciferase)1Smoc

SMOC番号

NM-KI-225025

維持形態

Sperm cryopreservation

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遺伝子の概要

Gene Symbol
Cd8a

説明

T2A-tdTomato-P2A-Luciferase expression cassette was knocked into the Cd8a gene .
応用分野:Reporter mouse

表現型データ

Fig.1.png

Fig.1 IVIS automatic exposure. According to the imaging process, the mice were injected with D-Luciferin, and the abdomen, back, and flanks were imaged in vivo. 

image.png

Fig.2 IVIS automatic exposure: ex vivo. According to the imaging process, D-Luciferin was injected, and the mouse brain, heart, lung, kidney, liver,  spleen, thymus, inguinal lymph node, axial lymph node (Axial LN), muscle, and the entire digestive system  (stomach to colon) were taken for imaging.

Fig.3.pngFig.3 Detection of tdTomato in mice thymus by FACS.

Fig.4.png

Fig.4 Detection of tdTomato in mice axillary lymph by FACS.

Fig.5.png

Fig.5 Detection of tdTomato in mice inguinal lymph by FACS.

Fig.6.pngFig.6 Detection of tdTomato in mice spleen by FACS.

Fig.7.pngFig.7 Detection of tdTomato in mice PB by FACS.

*The validation data above were completed in cooperation with Crownbio.


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