肝纤维化


胆管结扎肝纤维化小鼠模型

原理  通过结扎胆总管模拟胆道阻塞引起的肝纤维化模型 

样本要求  雄性C57小鼠7-8周龄

服务周期  18个工作日

技术指标  检测服务包括:1)血清检测:生化(ALT、AST、HA、ALB);  2)病理检测:HE、MASSON染色; 3)mRNA水平检测:Real-time PCR; 4)蛋白水平检测:Western blot 、FACS。

纤维化.png

图1. 胆管结扎示意图 


化学诱导肝纤维化小鼠模型

原理  四氯化碳可以对肝脏产生损伤,诱导肝纤维化(肝硬化)

样本要求  雄性C57小鼠8-10周龄

服务周期 适应3天,连续给药3W、6W、9W、12W、16W,根据给药不同周期,肝纤维化程度不同(根据具体实验要求调整)

技术指标  检测服务包括:1)血清检测:生化(ALT、AST、HA、ALB)、ELISA(ASPH);2)病理检测:HE、MASSON染色;3)mRNA水平检测:Real-time PCR;4)蛋白水平检测:Western blot 、FACS。


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